Spectrophotometry of proteins

Fluorescence Spectrophotometry

Fluorescence and phosphorescence are photon emission processes that occur during molecular relaxation from electronic excited states. These photonic processes involve transitions between electronic and vibrational states of polyatomic fluorescent molecules (fluorophores). The Jablonski diagram (Figure 1) offers a convenient representation of the excited state structure and the relevant transiti...

Spectrophotometry and Imaging of AGNs

We present low resolution spectrophotometric and imaging ISO observations of a sample of 58 AGN's over the 2.5-11.6 Jl range. The data strongly support unification schemes and set new constraints on models of the molecular torus.

Low-temperature spectrophotometry

Photoreactions of the fourth rhodopsin-like pigment, phoborhodopsin (pR480), of Hulobacrerium halobium were studied by low-temperature spectrophotometry. Upon irradiation of pR480 at -80 as well as 170°C with 436 nm light, a new intermediate having a difference absorption maximum at about 520 nm was produced. We designate this intermediate ‘P520’. Prolonged irradiation caused the formation of a...

Basic UV/Visible Spectrophotometry

Spectrophotometry of PRESAGETM polyurethane dosimeters

Spectrophotometry has been the conventional technique for measuring optical density of irradiated gels [1]. Later, magnetic resonance imaging (MRI) was found to be capable of imaging radiation dose patterns in 3D [2]. Optical methods are still under investigation in the form of optical tomography [3– 6]. They promise to give three dimensional dose distributions without the cost attached to MRI....